It is well accepted fact that cryo-preservation impairs sperm quality & viable sperm in number. Even after 60 years of continuous practice of sperm freezing in different species there is hardly much improvement in cryo-survival. Even till date the survival rate of sperm after freezing is 30-60%; It is little in difference from those 50 …
Presently like never before, Vitrification of semen is gaining popularity in research and discussion. Many researchers are working for vitrification of semen/sperm in their own specific manner. As a consequence, Myth & Misconception about Semen Vitrification is also blooming in the infertility industry. Till date, researchers working for semen vitrification are not unified. That is …
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Vitrification of semen gives an appropriate result in term of post thaw recovery and motility. Semen Vitrification is convenient & cost effective procedure. Moreover it gives very good progressive motility. In our ongoing work on Semen Vitrification without H.S.A., We noted some basic factors that influence semen vitrification and its outcome. Good quality semen …
We are in the continuous process to get best post-thaw motility of sperm after freezing by our newly developed vitrification method. Our main focus is on semen vitrification without using any animal component. This is my first attempt at sperm preparation following vitrification-thawing in boiling water. We decided to opt for single layer density gradient …
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Human semen freezing is almost six decades old technique with many up-gradation. Even after being such an old technique still Semen Freezing have many pitfalls. We will focus on the issue; how semen vitrification can fix most of freeze-thaw pitfalls?
We are working on whole semen freezing by technique of Vitrification. Semen freezing by Vitrification is still under trial. Hence Vitrified Semen Thawing is least discussed topic. We freezed three cryovials of each semen sample.. One vial in each collection remain control sample. We freeze control sample by conventional slow freezing method. Two more cryovials …
Vitrified Semen Thawing at 100 Degree C. & 45 Degree C… Read More »
Having baby is a dream of every family. Particularly if some one is desperate to get pregnant. Conception during Covid-19 pandemic, demands a comprehensive consideration. In true sense conception is initiation of a life and and continuation of blood-line. With conception every family or person think about normal pregnancy – child birth – up bringing …
With emerging new viruses, human semen and viral infections is a hot topic to discuss. The most common viral infection in human beings is influenza or common cold. Which spread is worldwide and almost whole population suffer twice or thrice in a year. It is more troublesome in cold countries and cold weather. In this …
Human Semen Vitrification, we first worked to vitrify semen (without washing) in November 2019. No doubt its was continuation of our previous work on sperm vitrification. As usual we had obstacles in beginning. Gradually we adjusted the concentration of components. As well we continuously reviewed whole protocol. Anyway now we are getting an acceptable and …
Fertility preservation is making its place with various indications. In fact fertility preservation is fertility insurance for future. With the advancement of science and emerging diseases, fertility preservation seems to be a rights for everyone. Moreover it must be 100 % safe from any future upcoming dangers. The recent technology of fertility preservation is freezing …
Safe Fertility Preservation should be Right to Everyone. Read More »
To our knowledge, this study invent First-Ever: Successful Vitrification of Human Sperm in an optimum quantity. Multiple attempts taken by many researchers to vitrify sperm of different living organism. But resulted either in partial success with controversy or failure. Moreover in our present work we got success in vitrifying human sperm (not semen) in an …
It is postulated that Ultra Rapid Freezing (Vitrification – ?) of Human Sperm require extremely high concentration of salt. Specially sucrose up to 1 mole or even more. My work on Ultra Rapid Freezing (Vitrification-?) of Human Sperm and Salt Concentration isn’t favoring this idea. Rather my work shows that even at adequate salt concentration …
Ultra Rapid Freezing (Vitrification-?) of Human Sperm and Salt Concentration. Read More »
Abstract — We designed an experiment to evaluate the effect and utility of H.S.A. in Human Sperm Vitrification. We compared post thaw recovery of sperm after vitrification with and without using H.S.A.. Also we had a control sample which was preserved by slow freezing method. Motility and progressive motility was main yardstick in our this …
Why we are lacking behind in the field of sperm preparation technique ? The aim should be to give better result. Not merely better business.
Successful vitrification of human sperm tried many times by researchers. But resulted only with partial success and with controversy or failure. This is first-ever successful vitrification of human sperm performed. Moreover quantity also remained in an optimum volume of 0.6 ml (600 micro liter). Which is appropriate for IUI, IVF or any infertility management. We …
The post “Effect of diet on sperm production & sperm quality” is a continuation of our previous post published on Linkedin around two year back. Which title was sperm quality and low calorie diet. This time we made the study “Effect of diet on sperm production & sperm quality” for a period of five weeks. …
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Numerous human sperm preservative media are in market. Many world leading companies are working to manufacture, upgrade and market human sperm preservative medium. But till date none of them worked to find or specify the ideal human sperm preservative medium and method. With this discussion we will simply try to enumerate the demerit of present human …
Ideal Human Sperm Preservative Medium and Method Read More »
Study on “Human Sperm preservation, with or without H.S.A. – A comparative study” is performed to know the role and necessity of H.S.A. for human sperm preservation. We conducted this study ( Human Sperm preservation, with or without H.S.A. – A comparative study ) by evaluating the outcome in term of post thaw motility, type …
Human Sperm Preservation with or without H.S.A. – A comparative study Read More »
After a long work we saw that L-Cystine in Semen hyper-viscosity management is significant enough. Especially L-Cystine in semen hyper viscosity management is valuable when you need to preserve the sperm from hyper-viscous semen. Semen coagulation Immediately after ejaculation semen coagulates. It is a normal phenomenon. The reason behind instant coagulation is not very clear. …
Cryopreservation of human sperm with H.S.A. is a standard method for preserving human sperm with various indications. We will discuss a exceptionally straightforward procedure in “Novel Technique to Cryopreserve Human Sperm without H.S.A. Which do not need any modern / expensive equipment. By The technique “Novel Technique to Cryopreserve Human Sperm without H.S.A.” our aim …
Novel Technique to Cryopreserve Human Sperm without H.S.A. Read More »
Current status of Vitrification of human sperm is either not clear or even we can say it isn’t helpful for IUI or IVF. By and by Vitrification of Oocyte, zygote, embryo and blastocyst is ordinarily performed to treat infertility. In present theme “Current status of Vitrification of human sperm” we will talk about present situation …
Make IUI Safe & More Productive We opted the topic “Make IUI safe & more productive, because IUI is performed much frequently than IVF. There is no clear data regarding the ratio of IUI & IVF. But there is estimation that 16.4 IUI is performed per IVF. There are two distinct reason behind this high …
Impact Of Humidity And Temperature On Human Sperm The point of composing the article ” Impact Of Humidity And Temperature On Human Sperm ” is to play out an analysis of human sperm creation with regular seasonal variation. As well as appraisal of variation in sperm quality and amount with the adjustment in temperature and …
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Sperm Preparation for ART —- Why & How When first Sperm Preparation for ART started? It is not very clear. According to available data first IUI started about a century ago, initially in cattle. First Assisted reproductive technology (ART) baby “Louise Brown” took birth on 25 July 1978. Following his birth, scope and future expectation …
Blood Group of IUI-D Born Child Blood group of IUI-D born child is also determined genetically as it happen in normally born child. In most of the case ABO Rh blood type is calculated & predicted according to child blood type calculator. Occasionally Blood Group of IUI-D born child is being influenced by some specific …