Den 1

Den – 1 is a single use kit having one vial of 2 ml (75 %) density gradient and 5 ml of washing media (Enhance ). It is being prepared to be stable up to 18 month. Its density gradient is silane coated colloidal silica particle along with HEPES based salts , which is being optimized at 75 % of density.
  • Most commonly used density gradient for sperm separation
  • Contain – 2 ml (75%) density gradient  +  5 ml washing media
  • Single use kit
  • Antioxidant —- Taurine
  • Antibiotic —- Gentamicin
  • Nutrition —– Albumin & Amino acids

Sperm washing for IUI

Sperm washing is the process which prepares a semen sample for IUI or IVF. This process effectively remove dead sperm and concentrate the sperm into a small volume which can easily be handled by the uterus.


  • Color — Hazy but clear
  • PH — 7.2 to 7.4
  • Density — 1.08
  • Sterility — sterile filtered
  • Sperm survival — 80% survival after 45 minutes of exposure with Den -1
  • 75% survival after 24 hours of exposure with Enhance

Packing & Self Life

  • Single use kit ( Pack of 20 kits)
  • Packed in borosilicate glass vial with silicon cap
  • Custom pack is available
  • Self life — 18 month


  • store Den – 1 kit between 2 – 8 Degree C
  • Take out only the required number of Den – 1 kits to keep in incubator
  • Use the Den – 1 kit under aseptic condition
  • Do not freeze.
  • The product can not be re-sterilized.
  • The product are stable during transport at higher temperature ( 37 Degree C) for 7 days.

Pre use check

  • Do not use the media if seal is open
  • Do not use if color has changed , there is any flocculation or any suspended particle.

Please Note

  • Mix Den – 1 vial by vial inversion 5 times before use.
  • To get the best result use Den – 1 at 32 Degree – 34 Degree C (not 37 Degree C)
  • Take upto 2 ml of semen sample to be washed by one kit of Den – 1.
  • Not for injection.
  • Allergies to antibiotics & albumin must be considered before IUI.
  • Sperm washing by density gradient can be used to decrease the risk of HIV transmission in HIV-positive males, since sperm washing reduce the virus load. However, there is no 100% guarantee that washed sperm is free from the HIV virus.

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