Numerous human sperm preservative media are in market. Many world leading companies are working to manufacture, upgrade and market human sperm preservative medium. But till date none of them worked to find or specify the ideal human sperm preservative medium and method. With this discussion we will simply try to enumerate the demerit of present human sperm preservative media and method. As well as we will also try our best to characterize ideal human sperm preservative medium and method.
Currently available sperm preservative medium is a mixture of many inorganic and some organic salts. It also contain sperm cell wall permeating and non permeating chemical substance to protect the sperm from damaging cryo-injury. The osmolarity (salt concentration) of human sperm preservative media is higher than any body fluid. Other than above salt sperm preservative medium also contain human serum albumin. Which complete composition is still not clear. Method of human sperm preservation is still more or less conventional. New equipment ( Programmable freezer, etc) has been tried to implement. At this point I would like to make it clear that temperature at vapor stage of liquid nitrogen can be reduced at any rate even manually. This is our practical experience that use of programmable freezer is only a way to sell this equipment. Certainly it is having its value in research. Otherwise it is of no use and simply increase the operational cost.
Demerits of conventional human sperm preservative compound and method.
Its composition is not uniform – every manufacturer is making some variation and claiming it best one.
All sperm preservative media contain human serum albumin.
Exact composition of human serum albumin is not clear. There is batch to batch and collection to collection variation in human serum albumin.
Human serum albumin is being obtained from another person. Therefore there is always a risk to transmit disease. Although human serum albumin supplier claim that they take all possible measures to supply disease free human serum albumin. But somewhere errors are unavoidable.
Method of human sperm preservation is getting costlier day by day. This is because of creating necessity of programmable freezer . Which is not a fact.
Every sperm preservation media manufacturer are advocating their own method to freeze human sperm.
Method of sperm preservation by freezing in liquid nitrogen is still cumbersome and time taking.
Thawing (bringing the sperm to normal temperature) protocol is not universal.
Requiredcharacteristics of ideal human sperm preservative medium and method.
In our day to day work, we noted that following measures should improve the preservation of Human sperm . As well as these measures will make the human sperm preservation easy, practical and safe.
Preservative compound must be 100 % chemically defined.
Freezing of human sperm should not require any costly equipment.
The method should be easy, acceptableand cost effective.
The composition of human sperm preservative medium should be simple and easy enough. How to prepare it anywhere should be documented. Which will drastically reduce the cost of human sperm preservation.
The time required should be minimum to perform preservation of human sperm.
Vitrification seems a way to make human sperm preservation easy, cost effective and practical.
Thawing should be done at a temperature of 32 degree C to 34 degree C. This is because development, maturation and storage of sperm is taking place at this temperature only.
Preserved sperm should be quarantined properly to prevent disease transmission from sperm donor side.
Discussion :— Preservation of human sperm is getting its popularity with increasing number of indications. It’s a well known fact that complications become more evident with increasing utility. Since the indications for human sperm preservation are increasing with different reasons. Accordingly the complication will increase and new complication will come forward. Our aim is to make human sperm preservation more and more perfect and complication free. In my opinion, above mentioned simple measures will result in an ideal human sperm preservative compound and method.
Vitrification is well known method to freeze human ovum and embryo. But till date none of the manufacturer has claimed successful vitrification of human sperm.
Conflict of Interest —- The topic “Ideal human sperm preservative medium and method” has not been published anywhere (as per our information). Therefore there is no conflict of interest.
Acknowledgements — Frozen Cell Team is in a nonstop procedure to find out an ideal media to preserve and process of human sperm. We are in an undertaking to find a better strategy to preserve and process human sperm by various procedure.
Funding — This post is solely prepared by Frozen Cell. No other organization or any system is not at all involved financially in any way for this post.
Limitations— We work with basic equipment and infrastructure. Therefore not able to provide sophisticated data.
26 thoughts on “Ideal Human Sperm Preservative Medium and Method”
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I just wanted to construct a simple remark to be able to say thanks to you for some of the great strategies you are giving out on this website. My long internet look up has finally been paid with reasonable know-how to write about with my close friends. I ‘d declare that most of us site visitors are very much lucky to dwell in a fabulous community with many lovely individuals with very beneficial strategies. I feel very grateful to have encountered the site and look forward to so many more fun minutes reading here. Thanks once again for a lot of things.
I must point out my passion for your generosity supporting persons who really want help with the area of interest. Your personal commitment to passing the message all around had become especially effective and has regularly permitted girls much like me to attain their desired goals. Your new valuable guideline signifies a lot to me and substantially more to my colleagues. Regards; from all of us.
My wife and i ended up being quite relieved when Albert could finish up his homework via the precious recommendations he came across from your own web page. It is now and again perplexing to just always be giving for free helpful tips some others might have been making money from. So we remember we’ve got the blog owner to be grateful to for that. All the illustrations you’ve made, the simple website menu, the relationships you can assist to foster – it’s all amazing, and it’s really assisting our son and us know that this idea is satisfying, which is unbelievably fundamental. Many thanks for the whole thing!
I simply wanted to make a quick comment to be able to express gratitude to you for the pleasant items you are sharing at this website. My rather long internet search has at the end of the day been recognized with wonderful know-how to share with my visitors. I would assume that most of us site visitors are unequivocally blessed to dwell in a useful website with very many brilliant individuals with useful advice. I feel quite fortunate to have seen the site and look forward to plenty of more pleasurable minutes reading here. Thanks a lot again for a lot of things.
I and my buddies were actually going through the best solutions located on the blog while quickly I had a horrible feeling I never expressed respect to the web site owner for those techniques. My guys were stimulated to see them and have seriously been taking advantage of them. We appreciate you indeed being so thoughtful and also for selecting variety of quality ideas most people are really wanting to learn about. Our sincere regret for not expressing gratitude to you earlier.
I just want to mention I am just beginner to blogging and certainly liked your web-site. More than likely I’m going to bookmark your blog post . You certainly have tremendous well written articles. Many thanks for sharing with us your web page.
My wife and i were absolutely relieved that Michael managed to do his research through the entire ideas he grabbed through your weblog. It is now and again perplexing just to find yourself releasing helpful hints that many a number of people may have been trying to sell. And now we discover we now have the website owner to thank because of that. The entire explanations you’ve made, the easy website menu, the friendships your site assist to create – it’s most powerful, and it’s leading our son and the family reason why the subject is amusing, and that’s unbelievably mandatory. Thank you for the whole thing!
My wife and i have been quite relieved that Albert could do his survey by way of the precious recommendations he discovered in your site. It is now and again perplexing just to choose to be releasing tips and hints which often some people might have been making money from. And we also discover we’ve got the writer to appreciate for this. The main explanations you made, the simple website menu, the relationships you can make it possible to engender – it’s all impressive, and it’s letting our son and the family do think this content is awesome, and that’s wonderfully vital. Many thanks for everything!
I as well as my guys were looking at the nice ideas from your website and then all of a sudden got an awful suspicion I had not thanked the web site owner for them. My men were definitely absolutely very interested to study them and have now without a doubt been having fun with them. Appreciation for really being indeed thoughtful and then for pick out variety of quality tips most people are really wanting to discover. Our own honest regret for not expressing gratitude to sooner.
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I simply wished to thank you so much once more. I’m not certain the things I would’ve gone through in the absence of those methods shared by you relating to my industry. It previously was a very troublesome difficulty in my view, but observing the very specialised avenue you treated the issue took me to cry over happiness. I am just happy for your advice and thus wish you really know what a powerful job that you’re getting into educating people using your websites. Most probably you have never encountered any of us.
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I not to mention my buddies were found to be looking at the nice solutions located on your website and quickly developed an awful suspicion I never expressed respect to the blog owner for those secrets. My boys had been totally happy to see all of them and already have surely been making the most of those things. We appreciate you actually being well kind as well as for deciding upon this kind of notable guides millions of individuals are really needing to be informed on. My personal honest apologies for not expressing appreciation to sooner.
My spouse and i ended up being quite excited when Michael could finish off his inquiry through your ideas he had out of the weblog. It’s not at all simplistic to just happen to be giving freely secrets which often other people have been trying to sell. And now we figure out we’ve got the website owner to appreciate for this. The specific explanations you have made, the easy web site navigation, the relationships you will give support to foster – it is mostly powerful, and it is leading our son and our family know that this article is satisfying, and that is highly important. Thanks for the whole thing!
I wish to express my affection for your generosity supporting all those that should have help on this subject matter. Your personal commitment to passing the message across ended up being wonderfully interesting and have frequently helped guys much like me to arrive at their ambitions. Your own valuable advice denotes much a person like me and somewhat more to my office colleagues. Regards; from each one of us.
I wanted to post a simple message so as to say thanks to you for all the pleasant tips and tricks you are sharing here. My extended internet lookup has now been honored with wonderful ideas to write about with my contacts. I would mention that we website visitors are unequivocally lucky to live in a fabulous site with many marvellous professionals with insightful suggestions. I feel rather privileged to have encountered your web page and look forward to so many more excellent moments reading here. Thanks once again for all the details.
I needed to post you one little bit of note in order to say thanks a lot over again with your incredible methods you’ve contributed above. It is certainly strangely generous with you to grant without restraint what a lot of people could have offered for sale for an electronic book in making some cash for themselves, certainly considering the fact that you could have tried it if you ever decided. These suggestions likewise acted like a great way to know that other people online have the identical zeal similar to my personal own to figure out a whole lot more with regard to this condition. I believe there are many more enjoyable sessions in the future for people who read through your site.
My husband and i felt really contented when Louis managed to do his inquiry through the entire ideas he discovered through your blog. It’s not at all simplistic just to always be giving for free points which usually many people may have been making money from. We really realize we have got the website owner to give thanks to for this. These explanations you made, the easy website menu, the relationships you will make it easier to foster – it’s got most unbelievable, and it’s really making our son and our family do think that subject is excellent, which is truly fundamental. Thank you for all!
I am only writing to make you understand what a brilliant encounter my princess obtained going through your web page. She discovered many details, with the inclusion of how it is like to have an amazing helping style to let certain people effortlessly fully understand selected very confusing subject areas. You actually did more than our expectations. I appreciate you for displaying such insightful, trusted, revealing and unique thoughts on this topic to Janet.
I precisely desired to appreciate you again. I am not sure what I could possibly have done in the absence of the entire solutions provided by you on such a subject. Entirely was a real troublesome issue in my opinion, nevertheless looking at the very skilled strategy you solved the issue made me to leap with delight. I’m just thankful for the information and as well , have high hopes you really know what a powerful job you were putting in training men and women using your blog. I know that you have never encountered any of us.
I would like to show my respect for your kind-heartedness giving support to folks that have the need for guidance on this important area. Your personal dedication to passing the solution across was really invaluable and have in every case enabled guys and women like me to arrive at their pursuits. Your new insightful help denotes this much a person like me and even more to my colleagues. Warm regards; from each one of us.
I intended to put you a very small note just to give many thanks the moment again for your exceptional views you’ve shared above. It has been really incredibly generous of people like you to allow unreservedly what exactly some people could possibly have supplied for an e-book to get some money for their own end, especially now that you might have tried it if you ever wanted. The principles as well served as a easy way to be aware that other people online have the identical interest much like mine to learn a good deal more in regard to this condition. I am certain there are a lot more pleasant occasions ahead for folks who look into your site.
I just wanted to construct a simple remark to be able to say thanks to you for some of the great strategies you are giving out on this website. My long internet look up has finally been paid with reasonable know-how to write about with my close friends. I ‘d declare that most of us site visitors are very much lucky to dwell in a fabulous community with many lovely individuals with very beneficial strategies. I feel very grateful to have encountered the site and look forward to so many more fun minutes reading here. Thanks once again for a lot of things.
I must point out my passion for your generosity supporting persons who really want help with the area of interest. Your personal commitment to passing the message all around had become especially effective and has regularly permitted girls much like me to attain their desired goals. Your new valuable guideline signifies a lot to me and substantially more to my colleagues. Regards; from all of us.
My wife and i ended up being quite relieved when Albert could finish up his homework via the precious recommendations he came across from your own web page. It is now and again perplexing to just always be giving for free helpful tips some others might have been making money from. So we remember we’ve got the blog owner to be grateful to for that. All the illustrations you’ve made, the simple website menu, the relationships you can assist to foster – it’s all amazing, and it’s really assisting our son and us know that this idea is satisfying, which is unbelievably fundamental. Many thanks for the whole thing!
I simply wanted to make a quick comment to be able to express gratitude to you for the pleasant items you are sharing at this website. My rather long internet search has at the end of the day been recognized with wonderful know-how to share with my visitors. I would assume that most of us site visitors are unequivocally blessed to dwell in a useful website with very many brilliant individuals with useful advice. I feel quite fortunate to have seen the site and look forward to plenty of more pleasurable minutes reading here. Thanks a lot again for a lot of things.
I and my buddies were actually going through the best solutions located on the blog while quickly I had a horrible feeling I never expressed respect to the web site owner for those techniques. My guys were stimulated to see them and have seriously been taking advantage of them. We appreciate you indeed being so thoughtful and also for selecting variety of quality ideas most people are really wanting to learn about. Our sincere regret for not expressing gratitude to you earlier.
I just want to mention I am just beginner to blogging and certainly liked your web-site. More than likely I’m going to bookmark your blog post . You certainly have tremendous well written articles. Many thanks for sharing with us your web page.
My wife and i were absolutely relieved that Michael managed to do his research through the entire ideas he grabbed through your weblog. It is now and again perplexing just to find yourself releasing helpful hints that many a number of people may have been trying to sell. And now we discover we now have the website owner to thank because of that. The entire explanations you’ve made, the easy website menu, the friendships your site assist to create – it’s most powerful, and it’s leading our son and the family reason why the subject is amusing, and that’s unbelievably mandatory. Thank you for the whole thing!
My wife and i have been quite relieved that Albert could do his survey by way of the precious recommendations he discovered in your site. It is now and again perplexing just to choose to be releasing tips and hints which often some people might have been making money from. And we also discover we’ve got the writer to appreciate for this. The main explanations you made, the simple website menu, the relationships you can make it possible to engender – it’s all impressive, and it’s letting our son and the family do think this content is awesome, and that’s wonderfully vital. Many thanks for everything!
I as well as my guys were looking at the nice ideas from your website and then all of a sudden got an awful suspicion I had not thanked the web site owner for them. My men were definitely absolutely very interested to study them and have now without a doubt been having fun with them. Appreciation for really being indeed thoughtful and then for pick out variety of quality tips most people are really wanting to discover. Our own honest regret for not expressing gratitude to sooner.
A lot of thanks for your entire hard work on this web site. My mum enjoys setting aside time for investigations and it is easy to see why. I hear all regarding the compelling method you provide efficient secrets via the web blog and in addition boost contribution from visitors on the concern and our favorite daughter is without question discovering so much. Take pleasure in the rest of the year. You’re performing a wonderful job.
I want to show my thanks to this writer just for rescuing me from such a challenge. Because of scouting through the search engines and coming across ways which are not powerful, I figured my life was well over. Existing without the presence of approaches to the issues you’ve fixed through this short article is a serious case, as well as ones which might have in a wrong way affected my career if I had not come across your site. Your main know-how and kindness in taking care of a lot of things was excellent. I am not sure what I would have done if I had not discovered such a point like this. I can at this point relish my future. Thanks for your time very much for your high quality and sensible help. I won’t be reluctant to recommend your web sites to anyone who requires recommendations on this subject.
I am glad for commenting to make you understand of the fine experience our child went through visiting your blog. She figured out lots of things, which include how it is like to possess an ideal coaching style to get the mediocre ones really easily comprehend a number of grueling issues. You truly surpassed my expected results. Thank you for producing these warm and friendly, trustworthy, revealing and also fun tips on this topic to Tanya.
I simply wished to thank you so much once more. I’m not certain the things I would’ve gone through in the absence of those methods shared by you relating to my industry. It previously was a very troublesome difficulty in my view, but observing the very specialised avenue you treated the issue took me to cry over happiness. I am just happy for your advice and thus wish you really know what a powerful job that you’re getting into educating people using your websites. Most probably you have never encountered any of us.
I have to express my appreciation to you just for rescuing me from this type of incident. As a result of looking through the world-wide-web and coming across principles that were not productive, I believed my life was done. Existing without the presence of answers to the difficulties you have resolved as a result of this post is a critical case, and the kind that could have in a negative way affected my entire career if I had not discovered your blog. The skills and kindness in maneuvering all the stuff was excellent. I don’t know what I would have done if I hadn’t encountered such a step like this. I am able to now look forward to my future. Thanks very much for your high quality and sensible guide. I will not be reluctant to propose the website to any individual who desires guidance on this subject.
I not to mention my buddies were found to be looking at the nice solutions located on your website and quickly developed an awful suspicion I never expressed respect to the blog owner for those secrets. My boys had been totally happy to see all of them and already have surely been making the most of those things. We appreciate you actually being well kind as well as for deciding upon this kind of notable guides millions of individuals are really needing to be informed on. My personal honest apologies for not expressing appreciation to sooner.
My spouse and i ended up being quite excited when Michael could finish off his inquiry through your ideas he had out of the weblog. It’s not at all simplistic to just happen to be giving freely secrets which often other people have been trying to sell. And now we figure out we’ve got the website owner to appreciate for this. The specific explanations you have made, the easy web site navigation, the relationships you will give support to foster – it is mostly powerful, and it is leading our son and our family know that this article is satisfying, and that is highly important. Thanks for the whole thing!
I wish to express my affection for your generosity supporting all those that should have help on this subject matter. Your personal commitment to passing the message across ended up being wonderfully interesting and have frequently helped guys much like me to arrive at their ambitions. Your own valuable advice denotes much a person like me and somewhat more to my office colleagues. Regards; from each one of us.
I constantly spent my half an hour to read this webpage’s articles or
reviews everyday along with a cup of coffee.
I wanted to post a simple message so as to say thanks to you for all the pleasant tips and tricks you are sharing here. My extended internet lookup has now been honored with wonderful ideas to write about with my contacts. I would mention that we website visitors are unequivocally lucky to live in a fabulous site with many marvellous professionals with insightful suggestions. I feel rather privileged to have encountered your web page and look forward to so many more excellent moments reading here. Thanks once again for all the details.
I needed to post you one little bit of note in order to say thanks a lot over again with your incredible methods you’ve contributed above. It is certainly strangely generous with you to grant without restraint what a lot of people could have offered for sale for an electronic book in making some cash for themselves, certainly considering the fact that you could have tried it if you ever decided. These suggestions likewise acted like a great way to know that other people online have the identical zeal similar to my personal own to figure out a whole lot more with regard to this condition. I believe there are many more enjoyable sessions in the future for people who read through your site.
My husband and i felt really contented when Louis managed to do his inquiry through the entire ideas he discovered through your blog. It’s not at all simplistic just to always be giving for free points which usually many people may have been making money from. We really realize we have got the website owner to give thanks to for this. These explanations you made, the easy website menu, the relationships you will make it easier to foster – it’s got most unbelievable, and it’s really making our son and our family do think that subject is excellent, which is truly fundamental. Thank you for all!
I am only writing to make you understand what a brilliant encounter my princess obtained going through your web page. She discovered many details, with the inclusion of how it is like to have an amazing helping style to let certain people effortlessly fully understand selected very confusing subject areas. You actually did more than our expectations. I appreciate you for displaying such insightful, trusted, revealing and unique thoughts on this topic to Janet.
I precisely desired to appreciate you again. I am not sure what I could possibly have done in the absence of the entire solutions provided by you on such a subject. Entirely was a real troublesome issue in my opinion, nevertheless looking at the very skilled strategy you solved the issue made me to leap with delight. I’m just thankful for the information and as well , have high hopes you really know what a powerful job you were putting in training men and women using your blog. I know that you have never encountered any of us.
I would like to show my respect for your kind-heartedness giving support to folks that have the need for guidance on this important area. Your personal dedication to passing the solution across was really invaluable and have in every case enabled guys and women like me to arrive at their pursuits. Your new insightful help denotes this much a person like me and even more to my colleagues. Warm regards; from each one of us.
“This site was… how do you say it? Relevant!! Finally I’ve found something that helped me. Cheers!”