Den – 2 is a single use kit having one vial of 1 ml (90%) & 1 ml (45%) density gradient and 5 ml of Enhance. Density gradient is silane coated colloidal silica particle with HEPES based salts , which is being optimized at 90% and 45% of density.
  • Most advanced used method for sperm separation
  • Contain – 1 ml of (90%) and I ml of (45%) density gradient & 5 ml washing media
  • Single use kit
  • Antioxidant —- Taurine
  • Antibiotic —- Gentamicin
  • Nutrition —– Albumin & Amino acids

Sperm washing for IUI

Sperm washing is the process which prepares a semen sample for IUI or IVF. This process effectively remove dead sperm and concentrate the sperm into a small volume which can easily be handled by the uterus.


  • Color — Hazy but clear
  • PH — 7.2 to 7.4
  • Density — 1.09 & 1.05
  • Sterility — Sterile filtered
  • Sperm Survival — 80% survival after 45 minutes of exposure with Den -2
  • 75% survival after 24 hours of exposure with Enhance

Packing & Self Life

  • Single use kit ( Pack of 20 kits)
  • Packed in borosilicate glass vial with silicon cap
  • Custom pack is available
  • Self life — 18 month


  • Store Den – 2 kit between 2 – 8 degree C
  • Take out only the required number of Den – 2 kits to keep in incubator
  • Use the Den – 2 kit under aseptic condition
  • Do not freeze.
  • Once the Den -2 vial has been opened or punctured , use all or discard.
  • The product can not be re-sterilized.
  • The product are stable during transport at higher temperature ( 37 Degree C) for 7 days

Pre use check

  • Do not use the media if seal is open
  • Do not use if color has changed , there is any flocculation or any suspended particle.

Please Note

  • Mix Den – 2 vial by vial inversion 5 times before use.
  • To get the best result use Den – 2 at 32 degree – 34 degree C (not  at 37 degree C)
  • Take up to 2 ml of semen sample to be washed by one kit of Den – 2.
  • Not for injection.
  • Allergies to antibiotics & albumin must be considered before IUI.
  • Sperm washing by density gradient can be used to decrease the risk of HIV transmission in HIV-positive males, since sperm washing reduce the virus load. However, there is no 100% guarantee that washed sperm is free from the HIV virus.

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