Impact Of Humidity And Temperature On Human Sperm
The point of composing the article ” Impact Of Humidity And Temperature On Human Sperm ” is to play out an analysis of human sperm creation with regular seasonal variation. As well as appraisal of variation in sperm quality and amount with the adjustment in temperature and stickiness (humidity). The outcome is being assessed in term of cryo-preservation and post thaw recovery.
This study is a dynamic of investigation of continuous 4 years of sperm cryo-preservation at distinctive months of year (from 2011 to 2014) in Delhi , capital of India.
Capital of India, Delhi has a wide variety in climate in the term of temperature (from 20 C in winter to 450 C in summer) just as dampness. In the equivalent way humidity likewise shifts from 2 to 80% without rain. In Delhi, substantial rain is moderately uncommon and stickiness (humidity) contacts 100 percent some times in a year as it were.
For the reason for study we have partitioned the year in three parts –
-
November , December , January & February – winter
-
March, April , May , June – summer
-
July , August, September & October – Autumn/Spring
Additionally we started cryo-preservation near a desert for a total year. It’s outcome has been thought about for end.
Distinctive sorts of studies have been done on animals and sea-going life by various researchers, which has been considered too.
For consistently six to eight donors were chosen for study. Every year an alternate gathering of donors was chosen for study. Semen was collected by masturbation. And donors was asked to come in morning only (From 7 AM to 10 AM). Additionally we ask the donors to not come void stomach. Temperature of the holding up corridor and accumulation room was kept in agreeable zone (320 C to 340 C).
All the handling / processing of semen was performed at temperature 320 C to 340 C
FINDINGS
A/ November , December, January and February (Winter – Low in temperature and low humidity)
In these months temperature fluctuates from 20 C to 250 C, Humidity is moderately low – 5 to 60%, (Humidity goes up if there should be an occurrence of fog or rain) .
-
Semen Analysis – good quality , morphological abnormal sperms was average (30 – 50). Total sperm count – 100 million to 250 million. Motility -50 to 70 %
-
Consequence of Cryo-preservation was great enough. Average number of cryo-preserved sperm vials prepared – 1-3 samples per collection.
-
Post thaw recovery good. Rejection based on post thaw recovery -10 – 25 %
-
Post thaw recovery – 15 % to 60 %
-
Post thaw motility % — from – 40% to 65 %
B/ March, April , May , June (Summer)
In these months, temperature changes from 300 C to 450 C. Most of the time dampness (humidity) stays on lower side – 2 to 40 %.
-
Semen Analysis – very great quality, morphological abnormal sperms was normal in count (30 – 45%) yet not exactly winters. Total sperm count – 125 million to 250 million, Motility -50 to 80 %
-
Aftereffect of cryo-preservation was moderately good, normal number of cryo-preserved sperm vials prepared – 2 – 4 samples per collection.
-
Post thaw recovery was great, post thaw rejection was somewhere – 5 – 15 %
-
Great post thaw recovery – 40 % to 90 %
-
Post thaw motility % – between – 50% to 70 %
C/ July , August, September and half of October (hot and sticky)
In these months for the most part temperature remain somewhere close to 300 C to 420 C. Most of the time stickiness stays on higher side from 60 % to 90 %.
-
Semen Analysis – Relatively poor quality of sperm by similar donors. Morphological irregular sperms was additionally on higher side (30 to 70%), Total sperm count – 60 million to 150 million , motility -40 to 70%
-
Consequence of cryo- preservation result was poor compared to other months. Average number of cryo-preserved sperm vials prepared – 0 – 2 samples per collection.
-
Post thaw recovery – poor. Rejection on post thaw recovery ground – 30 to 60%
-
Post thaw recovery – 25 to 50%
-
Post thaw motility – 20 to 60%
SPERM PRESERVATION NEAR DESERT
Our organization started a satellite cryo-preservation center around at 30 kilometer aerial distance from Jaislmer (a desert). Where temperature stays on higher side amid 9 months of the year (350 C +). Most vitally, humidity stays more often than not on lower side ( under 50%) . Only December , and January remain moderately cold (temp under 200 C.) But humidity stays on lower side. Rain is meager in this locale.
In this zone, sperm cryo-conservation performed from March 2015 to May 2016. Complete 26 donors drew nearer to join, out of 26. Out of 26, only 21 selected. Out of 21 just 9 proceeded for entire course of study.
Nature of the sperm , even the aftereffect of cryo-preservation was good and most importantly steady in quality.
-
Semen Analysis – very great quality, morphological abnormal sperms were normal (30 – 40%) Total sperm count – 1.5 million to 3.5 million, Motility -50 to 80 %.
-
Cryo-preservation result remained very good. Average number of cryo-preserved sperms vials prepared – 3 – 4 samples per collection.
-
Recovery post thaw – 30 % to 90 %
-
Post thaw motility % – 50% to 70 %.
-
Rejection post thaw -10 – 15 %
Essentially we noticed in this close desert zone –
-
Total sperm count was on higher side of all donors.
-
Out of 26 donors just a single donor was azoospermic and four were oligospermic, Which ( as per our comparative study) was less in contrast with the other part of the nation.
CONCLUSION
In the wake of concentrate all the outcome we concluded up
-
Whenever “Humidity and temperature” when both are available, it influence unfavorably for sperm production and sperm cryo-preservation.
-
Low temperature and high humidity does not influence the sperm production and cryo-preservation to that extent .
-
We got the best result with a temperature 300 C to 360 C( high temperature) and low humidity 5 % to 35 %. It demonstrate that just high temperature or just high humidity does not influence sperm production / cryo-preservation alone. We can finish up here, high temperature and high humidity , when the two components coincide , it influence the sperm creation antagonistically.
-
In our examination close desert we got the best outcome consistently, where humidity stays low consistently.
-
In adjacent desert zone, it appears that the rate of azoospermia and oligospermia are generally low. Further investigation is required regarding this perception.
-
Extraordinary of temperature ( under 20 C or more than 440C influences the sperm creation unfavorably. Particularly this affect was extreme when there was sudden change in the temperature.
-
In this examination I didn’t get much effect of day-light length on sperm production.
Numerous researches has led this kind of study on cows , pig, and so on. Almost all of them concluded that the moistness and temperature both act together and adversely affect sperm morphology & production.
This is useful study as a device for therapeutic the travel industry upgrade for fruitless couple. A couple with oligospermic husband can be encouraged to remain in such a zone where stickiness is low and temperature is between 300 C to 360 C.
Our organization are in process to do a further study. Where we will focus on impact of temperature and dampness in female. Where we will discuss on ova generation and rate of origination.
REFERENCES
- Effect of temperature and humidity on sperm morphology in boars under different housing systems in Thailand on boar semen — https://www.ncbi.nlm.nih.gov/pubmed/16141664
- Effect of temperature and humidity on sperm production in Duroc boars under different housing systems in Thailand – http://www.sciencedirect.com/science/article/pii/S0301622604000181
- Investigation of the Seasonal Variations of Temperature and Relative Humidity on Fertility potential — http://www.cals.msstate.edu/students/research/darby_dillard_report.pdf
———————– XXXX ————————-