Impact Of Humidity  And  Temperature On Human Sperm

The point of composing the  article ” Impact Of Humidity  And  Temperature On Human Sperm ” is to play out an analysis  of human sperm creation with regular seasonal variation.    As well as appraisal of   variation  in sperm quality and amount with the adjustment in temperature and stickiness (humidity). The outcome is being assessed in term of cryo-preservation and post thaw recovery.

This  study is a dynamic of investigation of continuous 4 years of sperm cryo-preservation  at distinctive months  of year (from  2011 to 2014) in Delhi , capital of India.

Capital of India, Delhi  has  a wide variety in  climate in the term of temperature (from 20 C  in winter to 450 C in summer) just as dampness. In the equivalent way  humidity  likewise shifts  from   2 to  80%  without rain.  In Delhi, substantial  rain is  moderately uncommon and stickiness (humidity) contacts 100 percent some times in a year as it were.

For the  reason for study we have partitioned the year in three parts  –

  1. November , December , January  &  February –   winter

  2. March, April , May , June  – summer

  3. July , August, September &  October  –  Autumn/Spring

Additionally we started cryo-preservation  near a  desert for a total year.   It’s outcome has been thought about for end.

Distinctive sorts of studies have been done on animals  and sea-going  life  by various researchers, which has been  considered too.

For consistently six to eight donors were chosen for study. Every year an alternate gathering of donors was chosen for study. Semen was collected  by masturbation.   And donors was asked  to come  in morning  only (From 7 AM  to 10 AM). Additionally we ask  the donors  to not come void stomach.  Temperature of the holding up corridor and accumulation room was  kept  in agreeable zone (320 C  to 340 C).

All the handling / processing of semen was performed at temperature 320 C  to 340 C

FINDINGS

A/ November , December, January and  February (Winter – Low in temperature  and low humidity)

In these months temperature fluctuates from 20 C  to 250 C,  Humidity  is moderately low – 5  to 60%, (Humidity goes up  if there should be an occurrence of fog  or  rain) .

  • Semen Analysis  –   good  quality ,  morphological  abnormal sperms was average (30 – 50).    Total sperm count  –   100 million to 250 million.   Motility  -50  to 70 %

  • Consequence of Cryo-preservation  was great enough.                                                                                                   Average number of cryo-preserved sperm vials prepared  – 1-3 samples  per collection.

  • Post thaw recovery  good.  Rejection based on post thaw recovery   -10 – 25 %

  • Post thaw recovery – 15 %   to 60 %

  • Post thaw motility %    —    from – 40%  to 65 %

B/  March, April , May , June  (Summer)

In these months, temperature  changes from 300 C   to  450 C.  Most of the time dampness (humidity) stays on lower side – 2  to  40 %.

  • Semen Analysis –   very  great quality,  morphological  abnormal sperms was normal in count (30 – 45%)  yet not exactly winters.     Total sperm count  – 125 million to 250 million,  Motility  -50  to 80 %

  • Aftereffect of cryo-preservation  was moderately good,  normal number of cryo-preserved sperm vials prepared  – 2 – 4 samples  per collection.

  • Post thaw recovery was great,   post thaw rejection was  somewhere   – 5 – 15 %

  • Great post thaw recovery – 40  %   to 90 %

  • Post thaw motility %  –   between – 50%  to 70 %

C/  July , August,  September and half of October   (hot and sticky)

In these months for the most part temperature remain somewhere close to 300 C  to 420 C. Most of  the time stickiness stays on higher side from 60 %  to 90 %.

  • Semen Analysis –   Relatively  poor quality  of sperm by similar donors. Morphological  irregular sperms was  additionally on higher side (30  to 70%),    Total sperm count  – 60  million to 150  million , motility  -40  to 70%

  • Consequence of cryo- preservation result  was poor compared  to other months.  Average number of cryo-preserved sperm  vials  prepared  – 0 – 2 samples  per collection.

  • Post thaw recovery – poor.  Rejection on  post thaw recovery ground   – 30 to 60%

  • Post thaw recovery – 25  to 50%

  • Post thaw motility – 20 to 60%

SPERM PRESERVATION  NEAR  DESERT

Our organization started a satellite  cryo-preservation  center  around at 30 kilometer aerial distance from Jaislmer  (a desert).  Where temperature stays on higher side amid 9 months of the year (350 C +).   Most vitally, humidity stays more often than not on lower side ( under 50%) .  Only  December , and January  remain  moderately  cold (temp under 200 C.)   But  humidity stays on lower side. Rain is meager in this locale.

In  this zone, sperm cryo-conservation performed from March 2015 to May 2016. Complete 26 donors  drew nearer to join, out of 26.   Out of 26,  only 21  selected.   Out of 21 just 9 proceeded for entire course of study.

Nature of the sperm , even the aftereffect of cryo-preservation was good  and most importantly  steady in quality.

  • Semen Analysis –   very  great quality,  morphological  abnormal sperms were normal (30 – 40%) Total sperm count  – 1.5 million to 3.5 million, Motility  -50  to 80 %.

  • Cryo-preservation result  remained  very good.           Average number of cryo-preserved sperms vials  prepared  – 3 – 4 samples  per collection.

  • Recovery post thaw  – 30  %   to 90 %

  • Post thaw motility % – 50%  to 70 %.

  • Rejection post thaw    -10 – 15 %

Essentially  we noticed  in this close desert zone   –

  1. Total sperm count  was on higher side of all donors.  

  2. Out of 26 donors  just a single donor was azoospermic  and  four  were oligospermic, Which ( as per our comparative study) was less in contrast with  the other part of the nation.

CONCLUSION

In the wake of concentrate all the outcome we concluded up

  1. Whenever “Humidity and temperature” when both are available, it influence unfavorably for sperm production and sperm  cryo-preservation.

  2. Low temperature and high  humidity does not influence the sperm production  and cryo-preservation to that extent .

  3. We got the best result  with a temperature 300 C to 360 C( high temperature)  and  low humidity  5 %  to 35 %.  It demonstrate that just high temperature or just high humidity  does not influence sperm production / cryo-preservation alone.  We can finish up here, high temperature and high humidity , when the two components coincide ,  it influence the sperm creation antagonistically.

  4. In our examination close desert we got the best outcome consistently, where humidity  stays low consistently.

  5. In adjacent desert zone, it  appears that the rate of azoospermia  and   oligospermia  are generally low.   Further investigation is required regarding  this perception.

  6. Extraordinary of temperature  ( under 20 C or more than 440C  influences the sperm creation unfavorably.  Particularly   this  affect was  extreme when there was sudden change in the temperature.

  7. In this examination I didn’t get much effect of day-light length on sperm production.

Numerous researches has led this kind of study on cows , pig, and so on.  Almost all of them concluded  that the moistness and temperature both act together  and  adversely  affect  sperm morphology  & production.

This is useful study  as a device for therapeutic the travel industry upgrade for fruitless couple. A couple with oligospermic  husband  can be encouraged to remain in such a zone where stickiness is low and temperature is between 300 C to 360 C.

Our organization are in process to do a further study. Where we will focus   on impact of temperature and dampness in female.   Where we will discuss on ova generation and rate of origination.

 REFERENCES

  1. Effect of temperature and humidity on sperm morphology in boars under different housing systems in Thailand on boar semen   —  https://www.ncbi.nlm.nih.gov/pubmed/16141664
  2. Effect of temperature and humidity on sperm production in Duroc boars under different housing systems in Thailand – http://www.sciencedirect.com/science/article/pii/S0301622604000181
  3. Investigation of the Seasonal Variations of Temperature and Relative Humidity on Fertility  potential   —     http://www.cals.msstate.edu/students/research/darby_dillard_report.pdf

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