Contain Sodium Chloride, Potassium Chloride, Sod. Lactate, EDTA, etc in tissue culture grade water to suit the cryopreservation of un-washed human semen. To facilitate cryopreservation of un-washed semen, HSA, Sucrose , Glycine & Glycerol is added in optimum quantity.

  • Antioxidant —- Taurine
  • Antibiotic —- Gentamicin

It does not contain any crude protein like egg yolk or milk powder.

Specification:

  • Color — Crystal Clear
  • PH — 7.2 to 7.4
  • Sterility — Sterile Filtered
  • Post thaw recovery — 20% to 60% depending upon sperm Quality
  • Sperm Survival — 80% after 45 minutes of exposure with with Pre wash C.P.

Packing Storage & Self Life:

  • 5 ml X 20
  • Packed in borosilicate glass vial with silicon cap
  • Custom pack is available
  • Self life — 6 month

Storage & Use

  • Store Pre wash C.P. at 2 – 8 Degree C
  • Take out only the required number of vials to keep in incubator
  • Use the vial under aseptic condition
  • Do not freeze.
  • Once the vial has been opened or punctured , use all or discard.
  • The product can not be re-sterilized
  • The product are stable during transport at higher temperature ( 37 Degree C ) for 7 days

Pre Use Check :

  • Do not use, if seal is open
  • Discard unused material
  • Do not use , if color has changed , there is any flocculation or any suspended particle.
  • To avoid contamination we strongly recommend that media should be opened and used only with aseptic technique.

BEFORE FREEZING:

  • Keep the Pre Wash C P , semen sample and all plastic disposable in incubator at 32 – 34 Degree C for 40 minutes. Let the semen liquefy completely.
  • In case of low sperm count it is preferable to concentrate the sperm before adding the Pre Wash C P
  • Add 1 ml of Pre Wash C P with 1.1 ml of liquefied semen sample. To avoid damage due to sudden osmotic change to the spermatozoa, the Pre wash C P should be added very slowly (drop wise) with continuous mixing of semen sample.
  • level the cryovials & make one QC cryovial
  • place the semen sample & prewash mixture in cryovials
  • keep the cryovials at room temperature 10 minute

HOW TO FREEZE:

  • Place the cryovials in a Freeze for 30 minutes (not in freezer compartment)
  • Keep cryovials at liquid nitrogen vapor stage, preferably 10 cm above the level of liquid nitrogen in a cryocan for 40 – 50 minutes.
  • Dip the cryovials in liquid nitrogen and check the post thaw count motility after 72 hours of cryopreservation.

PLEASE NOTE:

  • Freezibility is a factor, vary with individual and cryopreservation is directly dependent on freezibility.   Even very good quality semen sample could not be preserved if freezibility is poor.
  • Factors affecting freezibility – sperm maturity, sperm morphology, ROS, sperm antibody and unknown reason..

MUST KNOW

  • Freeze-­thawing caused a 32% to 37% reduction in normal morphological forms of sperm.
  • Sperm morphology, motility, mitochondrial activities and viability are equally susceptible to cryopreservation ­induced damage..
  • Once preserved with Pre wash C P , the IUI can be performed only after semen wash by Den – 1 or Den – 2.
  • Not for injection .

18 thoughts on “Pre Wash CP

  1. I precisely wanted to thank you so much all over again. I’m not certain the things that I would have tried in the absence of the actual secrets revealed by you concerning my subject. Certainly was a scary setting in my position, however , being able to view a well-written approach you dealt with the issue made me to weep with gladness. Extremely grateful for the advice and in addition have high hopes you recognize what a great job that you are undertaking educating many others using your blog post. I’m certain you’ve never come across any of us.

  2. I simply wanted to thank you so much all over again. I do not know the things that I could possibly have worked on without the entire thoughts shared by you on that subject. It absolutely was a real frightening problem for me, nevertheless encountering a well-written approach you handled that made me to jump with gladness. I will be thankful for the guidance and even believe you are aware of a great job you are putting in training some other people thru your web page. Most likely you haven’t met any of us.

  3. My wife and i have been happy when Jordan could deal with his inquiry out of the precious recommendations he obtained out of your web site. It is now and again perplexing to simply continually be giving out techniques which other people might have been selling. We fully grasp we have the writer to appreciate because of that. The main explanations you made, the easy website menu, the relationships your site aid to foster – it’s everything awesome, and it’s facilitating our son and us imagine that the article is brilliant, and that’s incredibly important. Thanks for the whole thing!

  4. I have to get across my appreciation for your kind-heartedness supporting those who actually need guidance on this one subject matter. Your personal commitment to passing the message along had become certainly advantageous and has without exception enabled somebody just like me to reach their aims. This warm and helpful information denotes a lot to me and a whole lot more to my colleagues. Many thanks; from all of us.

  5. Needed to put you one little bit of remark to help say thanks once again for those beautiful techniques you’ve featured on this website. It has been quite wonderfully open-handed with people like you to convey unreservedly just what most of us would’ve distributed as an e book in making some dough for themselves, especially considering that you might well have done it if you desired. The guidelines also acted like the good way to be certain that most people have a similar dreams just as mine to see a whole lot more regarding this problem. I’m sure there are several more fun moments up front for individuals who scan through your website.

  6. I wanted to post you this very small observation so as to thank you very much the moment again for those extraordinary secrets you have documented in this article. It is really tremendously open-handed of you to convey extensively all many of us might have supplied as an e-book to earn some bucks on their own, especially considering the fact that you might have tried it if you ever considered necessary. The inspiring ideas as well served like the easy way to recognize that some people have similar fervor like my own to know the truth way more with regards to this matter. I think there are thousands of more fun occasions in the future for folks who scan through your blog post.

  7. My husband and i felt really fortunate Jordan could finish up his basic research from your ideas he grabbed using your blog. It is now and again perplexing to just always be giving away procedures that many men and women might have been selling. We really already know we have got the blog owner to give thanks to because of that. The explanations you made, the easy website navigation, the friendships you will help instill – it’s got most remarkable, and it’s assisting our son and us understand this situation is excellent, which is certainly exceedingly essential. Thank you for everything!

  8. I simply had to appreciate you once again. I’m not certain the things I might have tried without these opinions discussed by you directly on my situation. It was actually a terrifying setting in my view, however , observing the skilled approach you solved that forced me to jump for contentment. I will be thankful for this help and then trust you realize what a powerful job you are doing educating the others through your webpage. I know that you have never encountered all of us.

  9. I simply desired to say thanks yet again. I am not sure what I would have handled in the absence of the type of recommendations contributed by you over this area. It became a terrifying matter in my opinion, nevertheless taking a look at the skilled technique you managed the issue took me to cry for joy. I am just thankful for your service and thus wish you know what a powerful job you have been undertaking training men and women with the aid of your web site. I’m certain you haven’t come across all of us.

  10. I simply wanted to jot down a brief note to thank you for those remarkable strategies you are placing at this site. My long internet investigation has finally been compensated with good details to talk about with my classmates and friends. I ‘d say that we readers actually are unquestionably endowed to live in a very good place with very many perfect individuals with very helpful basics. I feel rather fortunate to have encountered the site and look forward to tons of more entertaining minutes reading here. Thanks once again for all the details.

  11. I simply wanted to thank you so much once again. I am not sure what I could possibly have taken care of in the absence of the actual secrets revealed by you on such subject. It has been a real terrifying case in my position, however , being able to view this professional strategy you handled the issue took me to weep for joy. I will be thankful for this advice and thus believe you recognize what a powerful job that you’re getting into educating many others via your blog post. I know that you haven’t met all of us.

  12. A lot of thanks for your own effort on this web site. Betty take interest in going through internet research and it’s really easy to understand why. Most people learn all about the lively means you produce effective suggestions via the web site and in addition foster participation from some others about this issue so our simple princess is without a doubt starting to learn so much. Take advantage of the rest of the year. You’re conducting a brilliant job.

  13. My wife and i got quite joyous when Chris managed to round up his reports through the precious recommendations he was given when using the web page. It is now and again perplexing just to happen to be offering guidance that many others may have been trying to sell. So we acknowledge we have the website owner to appreciate for this. Those illustrations you’ve made, the easy site navigation, the relationships you will make it easier to foster – it’s got everything superb, and it’s letting our son in addition to our family feel that this topic is cool, and that’s truly fundamental. Thank you for everything!

  14. I am commenting to let you understand of the brilliant encounter my child gained checking your web page. She learned a lot of issues, which include what it is like to possess an ideal giving mindset to make other individuals very easily gain knowledge of selected hard to do subject areas. You undoubtedly did more than readers’ expected results. I appreciate you for providing such informative, trusted, edifying and also cool tips about the topic to Lizeth.

  15. I simply want to tell you that I’m all new to blogging and site-building and certainly loved your web blog. Most likely I’m planning to bookmark your blog post . You definitely have perfect articles. Thanks a bunch for sharing your website page.

  16. What’s up to every one, the contents present at this web site are really remarkable for people experience, well,
    keep up the good work fellows.

Leave a Reply

Your email address will not be published. Required fields are marked *