Contain NaCl, KCl, CaCl2, MgSO4, Sod. lactate, Taurine , Sod. Pyruvate, Glucose, Sod. Bicarb, HEPES, Al. Glutamine, EDTA, Albumin, Amino acids, Ph. red, Gentamicin, etc. in Tissue culture grade water.

  • HTF Designed media with HEPES.
  • Sperm survival up to 2 to 4 days.
  • Easy to use
  • Multiple use in IUI & IVF.
  • Single use pack of 5 ml.
  • Antioxidant —- Taurine
  • Antibiotic —- Gentamicin
  • Nutrition —– Albumin & Amino acids

Used As

  • Swim Up migration technique. (Direct & Not to direct)
  • Washing media in density gradient preparation.
  • Extension media of sperm before IUI or IVF as maintenance media.
  • Flushing media in IVF

Sperm washing for IUI

Sperm washing is the process which prepares a semen sample for IUI & IVF.

The swim up is used if the semen sample has a normal number of good sperm not too much debris .

By swim up technique, the sperms are selected on their motility and the capability to swim out of the seminal plasma.

Specification

  • Color — light rose color
  • PH — 7.2 to 7.4
  • Sterility — sterile filtered
  • Sperm survival — 75% survival after 45 minutes of exposure with Den-2
  • Survival up to 2 to 4 days

Packing & Self Life

  • Single use kit ( Pack of 20 kits)
  • Packed in borosilicate glass vial with silicon cap
  • Custom pack is available
  • Self life — 18 month

Storage

  • Store Enhance between 2 Degree – 8 Degree C
  • Take out only the required number of Enhance vials to keep in incubator
  • Use the Enhance under aseptic condition
  • Do not freeze.
  • Once the Enhance vial has been opened or punctured , use all or discard..
  • The product can not be re-sterilized.
  • The product are stable during transport at higher temperature ( 37 Degree C) for 7 days.

Pre Use Check

  • Do not use the media if seal is open
  • Do not use the media if color has changed , there is any flocculation or any suspended particle.

Please note

  • To get the best result use Enhance at 32 Degree – 34 Degree C ( not 37 Degree C )
  • Not for injection.
  • Allergies to antibiotics & albumin must be considered before IUI.
  • Sperm washing can be used to decrease the risk of HIV transmission in HIV-positive males, since sperm washing reduce the virus load. However, there is no 100% guarantee that washed sperm is free from the HIV virus.

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