We are working on whole semen freezing by technique of Vitrification. Semen freezing by Vitrification is still under trial. Hence Vitrified Semen Thawing is least discussed topic. We freezed three cryovials of each semen sample.. One vial in each collection remain control sample. We freeze control sample by conventional slow freezing method. Two more cryovials we freezed of same semen samples by method of vitrification.
The aim of this study is to compare the quality of post thaw motility of vitrified semen: When thawed at different temperature.
- By using conventional method of thawing: control
Cyrovial taken out from liquid nitrogen & kept in incubator at 330 C for 45 minutes.
- By thawing at 100 degree C (in boiling water)
After taking out from liquid nitrogen, cryovial was waved in the air for few second and finally plunged in boiling water.
- By thawing at 45 degree C
After taking out from liquid nitrogen, cryovial was dipped in water at 45O C
Material used: Post Wash CP to freeze one cryovial by conventional method., Pre Wash Vitri Media to vitrify semen sample, double walled cryovial, liquid nitrogen, etc.
After collection whole semen kept in incubator at 33 degree C for 45 minutes – till complete liquefaction.
One ml of Pre Wash Vitri Media slowly added to completely liquefied and mixed semen. Mixed regularly and gently. Filled in cryovials. Kept at room temperature for 15 minutes. All cryovials plunged in liquid nitrogen.
After 24 hour we thawed all cryovials. Control cryovial was thawed as usual being done routinely. For vitrified semen we used two different protocol of thawing.
Conclusion: Vitrified Semen Thawing
We did not notice any gross difference in both method of thawing. Thawing even at 100 degree C is equally good in case of vitrified semen. We compared with conventional post thaw recovery of semen. And our observation is relatively fast motility in vitrified sample. Which indicate somewhere that vitrified semen may give better result in term of conception
Moreover we understand, our study is limited to post thaw recovery of motility. Even sample size is small. But we can conclude with confidence that semen can be vitrified with successful post thaw recovery of motility.
——————– End of article — xxx
21 thoughts on “Vitrified Semen Thawing at 100 Degree C. & 45 Degree C…”
As the admin of this site is working, no hesitation very quickly it will
be famous, due to its feature contents.
Howdy are using WordPress for your blog platform? I’m new
to the blog world but I’m trying to get started and set
up my own. Do you require any coding knowledge to make your own blog?
Any help would be greatly appreciated!
Asking questions are genuinely nice thing if you are not understanding anything totally, however this piece of writing presents fastidious understanding even.
Hurrah! After all I got a website from where I know how
to genuinely obtain helpful facts concerning my study and knowledge.
Wonderful blog! I found it while browsing on Yahoo News.
Do you have any tips on how to get listed in Yahoo
News? I’ve been trying for a while but I never seem to get there!
I’m not that much of a internet reader to be
honest but your blogs really nice, keep it up!
I’ll go ahead and bookmark your site to come back later
on. Many thanks
The main thing is that everything is real, personally checked!
Good luck to everyone in the game!
What’s up, its fastidious article concerning media print, we
all understand media is a impressive source of information.
Its like you read my mind! You seem to know a lot about this, like you wrote the
book in it or something. I think that you can do with some pics to drive
the message home a bit, but other than that, this is magnificent blog.
A fantastic read. I’ll definitely be back.
Pretty section of content. I just stumbled
upon your weblog and in accession capital to assert that
I get actually enjoyed account your blog posts. Anyway I will be subscribing to your feeds
and even I achievement you access consistently quickly.
Thanks for your personal marvelous posting! I truly enjoyed reading it, you can be a great author.I will be sure to
bookmark your blog and will often come back from now on.
I want to encourage you to continue your great work, have a nice weekend!
guidelines to write a research paper
Hi there, i read your blog from time to time and i own a similar one and i
was just wondering if you get a lot of spam responses? If so how do you protect against it, any plugin or anything you
can advise? I get so much lately it’s driving me mad so any help is very much appreciated.
Hello to all, for the reason that I am genuinely
eager of reading this website’s post to be updated regularly.
It consists of pleasant stuff.
Hello there, just became aware of your blog through Google, and found that it’s
truly informative. I am gonna watch out for brussels.
I will appreciate if you continue this in future.
Many people will be benefited from your writing. Cheers!
I wish you good luck to become your permanent companion.
I loved as much as you’ll receive carried out right here.
The sketch is tasteful, your authored material stylish.
nonetheless, you command get bought an shakiness over that you wish be
delivering the following. unwell unquestionably come more formerly again as exactly the same nearly a lot often inside case you shield this increase.
Hi, i believe that i noticed you visited my blog thus
i got here to return the choose?.I’m trying to
in finding things to enhance my site!I guess its
ok to make use of a few of your ideas!!
Greetings! Very useful advice in this particular article! It’s the little changes that make the biggest changes.
Many thanks for sharing!
I seriously love your site.. Pleasant colors & theme. Did you create this amazing site
yourself? Please reply back as I’m looking to create my very own blog and
would love to learn where you got this from or exactly what the theme is named.
Thanks for the good writeup. It actually was once a leisure account it.
Glance advanced to more brought agreeable from you!
By the way, how can we keep in touch?