Human sperm preservation has been started in 1980es. Much earlier  sperm preservation was being done for cattle with the intention to improve  pedigree.  Later on sperm preservation carried on for other mammalians, aquatics and avian also with different motives. During initial days of Intra uterine insemination  (IUI) treatment only few disease was known as disease transmissible by IUI.  But now  disease transmission by IUI have a bigger list  that also include unidentified & emerging viruses  and  prions. 

Keywords – Human sperm preservation,  Sperm preservation, Sperm Cryo-preservation, disease transmission by IUI.  Unidentified & emerging viruses, Prions.

Source of infection  in IUI  —-

Direct  source  —–

  • Direct source are sperm donor /husband.
  • Sperm donor / husband can be properly screened before & after donation  for any period of time.
  • Sperm donor /husband remain in direct contact with clinician or Sperm Bank,  therefore relatively easy to screen.
  1. Direct  Source    —————
  • Direct source are sperm donor /husband.
  • Sperm donor / husband can be properly screened before & after donation for any period of time.
  • Sperm donor or husband remain in direct contact with clinician or Sperm Bank,  therefore relatively easy to access  and screen.
  1. Indirect  Source   ———–

  • Indirect source are serum fraction (human, animal or avian products) added in Cryo-preservative media to augment sperm preservation (cryo-preservation), nutrition, post thaw motility enhancement etc.
  • Now a day mainly “Human serum albumin” (HSA) is used for human sperm preservation (cryo-preservation). It is being purchased from companies which is  not in direct contact with user.  Surprisingly even manufacturing company are giving dubious statement about  infectivity or possibility of transmission of disease. Manufactures are not in condition to give full assurance that disease will not be transmitted  by used HSA.
  • Infertility clinic or clinician , who performs Human sperm preservation(cryo-preservation) do not have any direct control on quality of HSA. Even they can not cross check it.
  • Batch to batch variation in composition is common with HSA and  FBS
  • Rarely Fetus bovine serum (FBS) or egg yolk is used for Human sperm preservation (cryo-preservation). Zoonotic disease are coming up in sporadic manner all over world.  Over 200 diseases are currently classified as  zoonoses.   Intra Uterine Insemination by Human sperm preservation (cryo-preservation) may directly increase the incidence.
  • Serum is an ill defined  very complex mixture of large number of constituents  with multiple growth promoting & growth inhibiting activities
  • Serums contain different amount of endotoxins, hemoglobin & many other adverse factors  such as  bacteria, fungi,  viruses & prions.
  • Prions, if present with Bovine serum, can not be diagnosed or destroyed even at very high temperature .
  • Even for the manufacturing process of Vaccines many attempts are being taken to find a way to replace growth media containing bovine derived materials by “synthetic” media.
  • Freezing of sperm does not have any destructive effect on micro-organism / prions that is being freezed  along with sperm.

Purpose of discussion

The aim of this discussion is to point out theoretical & remote possibility of  disease transmission by IUI  through  human , animal or avian protein / serum.  Above all, possibility  of getting infected by unidentified & emerging viruses are always there.  For case of Prions all possible measures are being taken, but because of commercialization  & viability  bovine serum manufacturing units may think to import  the animals.  and infected serum with prions can be supplied in the market.

                My point is – When  human sperm preservation (cryo-preservation)  can be performed without the use of  HAS  or any serum,  why to use  HAS or  any serum.

How effectively human sperm preservation (cryo-preservation) can be done even without  HSA   can  seen by  given short clip of video.  The used cryo-preservation media does not contain any complex molecule like HSA, or any  human, animal or avian protein. It is 100 % chemically defined  sperm  preservative (cryo-preservative) media.  Since it does not contain HSA,  therefore its  half life is more and cold chain maintenance is not required.   It has been prepared simply by judicious use of amino acids and salts. Post thaw motility  recovery varies from 30 to 70 %. Progressive and straight forward motility is clearly visible  in the video clip.

Reference

1/    Medical Definition of Zoonotic.
2/ Alternatives to the Use of Fetal Bovine Serum:  Serum-free Cell Culture,  Gerhard      Gstraunthaler,  Department of Physiology, University of Innsbruck, A-Innsbruck
3/  Archives of Andrology, Journal of Reproductive Systems,  Transmission of Sexually Transmitted Diseases by  Donor Semen,  B. S. Shanis, J. H. Check & A. F. Baker
4/    The European Agency for the Evaluation of Medicinal Products, Evaluation of Medicines for    Human Use,  London, 24 April 2001 EMEA/CPMP/BWP/819/01

43 thoughts on “Why To Preserve Human Sperm with HSA ??

  1. I precisely needed to thank you very much once more. I do not know the things I might have followed without the tricks discussed by you concerning such a problem. Entirely was a real horrifying dilemma in my view, however , considering your expert way you solved the issue took me to jump for contentment. I’m just happy for the service and thus wish you comprehend what a great job that you are doing educating the rest with the aid of your webblog. Most probably you haven’t encountered all of us.

  2. I together with my pals were viewing the excellent tricks found on your web site and so unexpectedly came up with a horrible feeling I had not thanked the website owner for those strategies. Those guys are actually so joyful to study them and now have definitely been enjoying those things. Thank you for simply being so considerate and then for pick out such good information millions of individuals are really desirous to be aware of. My personal honest apologies for not expressing appreciation to you earlier.

  3. I really wanted to construct a simple message to be able to express gratitude to you for these remarkable ways you are showing at this site. My incredibly long internet look up has at the end been compensated with reasonable ideas to share with my partners. I would declare that many of us readers are quite endowed to be in a superb site with so many marvellous individuals with useful principles. I feel very happy to have seen the webpages and look forward to so many more fabulous times reading here. Thanks a lot once more for a lot of things.

  4. I am also writing to make you be aware of of the terrific encounter my wife’s child gained using the blog. She noticed lots of things, which included what it is like to have a very effective giving nature to let folks with ease learn certain hard to do topics. You really surpassed readers’ desires. I appreciate you for delivering these valuable, trusted, edifying and also unique thoughts on this topic to Evelyn.

  5. I wish to voice my love for your kindness giving support to persons that need assistance with this important idea. Your personal dedication to getting the solution across ended up being particularly informative and has really empowered people like me to achieve their ambitions. This interesting publication entails so much a person like me and a whole lot more to my peers. With thanks; from all of us.

  6. Hi just wanted to give you a brief heads up and let you know a few of the images aren’t loading properly.

    I’m not sure why but I think its a linking issue.
    I’ve tried it in two different internet browsers and both show the
    same results.

  7. hello!,I love your writing so so much! proportion we keep in touch extra approximately your post on AOL?
    I need an expert on this space to unravel my problem.
    May be that’s you! Having a look ahead to look
    you.

  8. I wish to express my admiration for your kind-heartedness supporting those individuals that should have help with this particular matter. Your personal commitment to getting the solution all around had become especially helpful and has really helped women much like me to reach their dreams. Your amazing interesting key points signifies so much a person like me and substantially more to my colleagues. Many thanks; from everyone of us.

  9. Wow, fantastic blog layout! How long have you ever been running a blog for?

    you make running a blog look easy. The whole look of your website is
    magnificent, as smartly as the content material!

  10. I wish to express my appreciation to this writer just for rescuing me from this particular situation. After checking through the internet and seeing basics that were not pleasant, I was thinking my entire life was done. Living minus the solutions to the difficulties you’ve solved by means of your good site is a serious case, as well as the ones which could have badly affected my entire career if I had not encountered the blog. The natural talent and kindness in dealing with every aspect was tremendous. I’m not sure what I would’ve done if I hadn’t come across such a stuff like this. I am able to at this moment relish my future. Thanks a lot so much for the specialized and result oriented guide. I will not think twice to recommend your site to anyone who should get guide on this topic.

  11. My husband and i were quite relieved Ervin managed to deal with his researching from the ideas he got when using the site. It’s not at all simplistic just to continually be giving for free solutions that many many others could have been selling. And now we understand we need you to appreciate for that. The most important explanations you’ve made, the easy blog menu, the friendships you will assist to foster – it’s mostly spectacular, and it is making our son in addition to our family believe that the situation is entertaining, which is certainly tremendously serious. Many thanks for all the pieces!

  12. I really love your site.. Very nice colors & theme. Did you make this
    amazing site yourself? Please reply back as I’m
    hoping to create my own personal website and want to find
    out where you got this from or just what the theme is named.

    Thanks!

  13. You really make it seem so easy with your presentation but I find this
    matter to be really something that I think
    I would never understand. It seems too complex and very broad for
    me. I am looking forward for your next post, I will try to get the hang of it!

  14. Thank you for any other informative website.
    Where else may I get that kind of info written in such an ideal means?
    I’ve a undertaking that I’m just now operating on, and I
    have been at the glance out for such info.

  15. I would like to express my thanks to you for bailing me out of this particular incident. As a result of exploring throughout the world-wide-web and meeting methods that were not helpful, I was thinking my life was done. Existing devoid of the approaches to the difficulties you’ve resolved as a result of your short post is a serious case, as well as those that would have adversely damaged my career if I hadn’t encountered the website. The know-how and kindness in taking care of all the pieces was priceless. I’m not sure what I would have done if I hadn’t come upon such a point like this. I can also at this time relish my future. Thanks a lot very much for your expert and sensible guide. I won’t hesitate to propose your site to anybody who would need direction on this subject matter.

  16. Thank you for your own effort on this website. Kim enjoys doing investigation and it’s easy to understand why. We notice all regarding the compelling form you deliver informative steps via the web blog and as well recommend participation from the others on this subject matter while our favorite daughter is in fact discovering a lot of things. Take pleasure in the remaining portion of the year. Your conducting a very good job.

  17. My spouse and i ended up being really peaceful when Raymond managed to finish up his research because of the precious recommendations he obtained from your web pages. It’s not at all simplistic to simply continually be giving for free helpful hints that men and women may have been trying to sell. Therefore we take into account we’ve got the writer to be grateful to for that. All of the explanations you’ve made, the easy site navigation, the friendships you can make it possible to instill – it is most astonishing, and it is leading our son and the family imagine that the concept is entertaining, and that’s seriously important. Many thanks for everything!

  18. I needed to post you that little bit of remark just to give many thanks once again over the nice methods you have discussed in this case. This is quite surprisingly open-handed with people like you to allow without restraint precisely what a number of us would have distributed as an ebook to get some cash on their own, precisely considering the fact that you could have tried it if you wanted. Those basics likewise worked to become great way to understand that other individuals have the identical interest similar to mine to realize more concerning this matter. I think there are thousands of more pleasant times up front for many who view your blog post.

  19. I am really impressed along with your writing abilities and also with the structure for your
    blog. Is that this a paid subject matter or
    did you modify it your self? Anyway keep up the excellent high quality writing, it’s uncommon to peer
    a great weblog like this one nowadays..

  20. I wanted to post you the bit of observation so as to thank you very much yet again for your fantastic solutions you have contributed in this article. It has been really remarkably generous of people like you to provide easily what exactly a number of us might have advertised as an e-book to get some money for themselves, notably considering that you might have tried it in case you decided. These guidelines likewise served to be a fantastic way to be sure that someone else have a similar eagerness really like my very own to know the truth more and more related to this problem. I know there are a lot more pleasant opportunities ahead for individuals that browse through your website.

  21. I must voice my affection for your kindness for men who need help with this one area of interest. Your personal dedication to getting the message throughout became definitely important and have usually allowed men and women much like me to attain their aims. Your entire insightful tips and hints means a whole lot a person like me and far more to my fellow workers. Thanks a ton; from all of us.

  22. My spouse and i ended up being quite peaceful when Peter managed to carry out his basic research through the precious recommendations he acquired through your web pages. It is now and again perplexing to simply possibly be offering solutions which usually the rest could have been selling. So we know we now have the website owner to thank because of that. The type of explanations you made, the simple site navigation, the relationships you will make it easier to instill – it is mostly sensational, and it is making our son and us know that the issue is interesting, and that is highly vital. Thank you for all!

  23. I actually wanted to send a brief remark in order to thank you for all of the unique tips and tricks you are giving at this website. My extended internet lookup has now been honored with sensible knowledge to share with my contacts. I would repeat that we site visitors actually are undeniably blessed to exist in a perfect community with very many marvellous professionals with good ideas. I feel somewhat fortunate to have used the site and look forward to so many more awesome times reading here. Thanks a lot once again for all the details.

  24. I enjoy you because of all your effort on this web page. Gloria loves getting into investigation and it is easy to see why. My spouse and i learn all regarding the lively medium you convey efficient tips and hints through your website and in addition increase contribution from other ones on that subject while our favorite child is always becoming educated so much. Take pleasure in the rest of the new year. Your conducting a superb job.

  25. My husband and i felt so satisfied when Albert managed to round up his preliminary research while using the ideas he came across when using the web pages. It is now and again perplexing just to always be offering information and facts most people might have been making money from. And we all understand we have got the website owner to thank for that. The most important illustrations you made, the straightforward website navigation, the friendships you can make it possible to foster – it is most remarkable, and it’s aiding our son and our family reason why this situation is fun, and that’s truly important. Thank you for the whole lot!

  26. Needed to write you that tiny note to give many thanks the moment again for the exceptional guidelines you’ve provided here. It is certainly particularly open-handed of people like you to make unreservedly all that many of us would have made available as an e book to generate some profit for themselves, even more so considering the fact that you could have tried it if you considered necessary. These tricks additionally served like a easy way to be certain that other individuals have the same passion just like my very own to figure out great deal more on the subject of this problem. I am certain there are many more pleasant instances ahead for people who look into your blog post.

  27. Needed to compose you that little note just to say thanks a lot once again for all the extraordinary tips you’ve discussed on this page. This has been extremely open-handed of you to grant unreservedly exactly what many of us would have distributed for an e-book to get some money on their own, precisely considering that you could possibly have done it in case you wanted. Those good tips in addition served to provide a great way to be certain that some people have the identical eagerness much like my personal own to grasp very much more when considering this matter. I am sure there are some more pleasurable situations in the future for those who look into your blog.

  28. I would like to point out my passion for your kind-heartedness for persons that have the need for help with this particular topic. Your very own dedication to getting the message all-around ended up being quite practical and have surely allowed individuals just like me to reach their goals. Your own important suggestions entails much to me and even further to my peers. With thanks; from each one of us.

Leave a Reply

Your email address will not be published. Required fields are marked *