Enhance without  H.S.A.

Products Without H.S.A. / By

Enhance without  H.S.A.

Contain NaCl, KCl, CaCl2, MgSO4, Calcium  lactate, Taurine , Sod. pyruvate, Glucose, Sod. bicarbonate, HEPES, Methyl cellulose, Alanyl  glutamine, EDTA, Amino acids, Phenol  red, Gentamicin, in tissue culture grade water.

  • Almost equal  to HTF
  • Sperm survival up to 1 to 3 days.
  • Multiple use in IUI.
  • Single use pack of 5 ml.

 

  • Antioxidant —- Taurine & Amino acid.
  • Antibiotic —- Gentamicin
  • Nutrition —–  Amino acids & Carbohydrate

 

Methyl cellulose :—
  • non-toxic,
  • non-allergenic,
  • non-carcinogenic,
  • non-infectious,
  • plant-derived macro-molecule
  • antioxidant
  • No osmolytic properties

 

Can be used As

  • Swim up migration technique. (direct & not to direct)
  • Washing media in density gradient preparation.
  • Preparation of Frozen Semen Sample.

 

Sperm washing without H.S.A  for IUI

  • Sperm washing is a process to prepares a sperm  for IUI & IVF. 
  • The swim up is used if the semen sample has a normal number of good sperm and not too much debris .
  • By swim up technique, the sperms are selected on their motility and the capability to swim out of the seminal plasma.

Specification

  • Color — light rose color
  • PH — 7.2 to 7.4
  • Sterility — Sterile Filtered
  • Sperm Survival — 75% Survival after 45 minutes.
  • Survival up to 1 to 3 days.

Packing & Self Life

  • Single use kit
  • Packed in borosilicate glass vial with silicon cap
  • Self life — 18 month

Storage, Use & Precautions.

  • Store  between 2 Degree – 8 Degree C.
  • Take out only the required number of  vials to keep in incubator.
  • Use  under aseptic condition.
  • Do not freeze.
  • Once the vial has been opened or punctured , use all or discard.
  • The product cannot be re-sterilized.
  • The product are stable during transport at higher temperature ( 37 Degree C) for 5 days.

Pre Use Check

  • Do not use the media if seal is open
  • Do not use the media if color has changed , there is any flocculation or any suspended particle.

Please note

  • To get the best result use at 32 – 34 Degree C (not 37 Degree C)
  • Check the motility after 10 minutes of incubation.
  • Not for injection.
  • Allergies to antibiotics  must be considered before IUI.
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