New Extender For Preservation Of Human Spermatozoa
The new extender for preservation of human spermatozoa is without albumin and not having any protein of any origin. It is 100% chemically defined hence can be prepared anywhere without any variation. I expect there is no need to consider about bad or unknown consequences of HSA by using this extender(when composition of HSA is not very clear).
Unlike conventional semen extender, the New Extender For Preservation Of Human Spermatozoa is without albumin. Hence does not contain any crude protein . Protein is a very complex molecule among all organic compounds. Source of albumin , used for spermatozoa preservation, is either human or animal. The newly developed New Extender For Preservation Of Human Spermatozoa does not contain albumin. Hence the New Extender For Preservation Of Human Spermatozoa does not have any bad effect of protein (know or unknown) on spermatozoa. http://www.frozencell.org/blog/2018/06/01/why-to-preserve-cryo-preserve-human-sperm-with-hsa-hsa-additional-source-of-disease-transmission/
Human sperm preservation medium
Sperm preservation has been started in 1960. Many form of protein used e.g. patient own plasma, egg yolk, milk protein, coconut water, bovine serum, human serum albumin, etc. For last two decades Human Serum Albumin is in common use as a component of semen extender for human. Presently human serum albumin is an integral a part of human sperm preservation medium.
Human sperm cryo-preservation
There are two method of Human SpermPreservation
Raw sperm cryo-preservation — In this method Human Sperm Cryo-preservation is done without washing the sperm or separating the sperm from seminal fluid, debris, dead sperm, epithelial cell, foreign body etc. After liquefaction of semen we add extender slowly with liquefied semen. This method require washing of thawed semen before insemination. This method have the benefit of preserving antioxidant of seminal plasma. Which helps in post thaw motility recovery of sperm. This method of semen cryopreservation is in very common use.
Washed sperm preservation — This is Human Sperm Preservation after washing. Most frequently we wash the the semen by density gradient method. Washing of semen effectively remove debris, dead sperm and foreign body, if any. Concentrated sperm comes in the form of a pellet. Followed by mixing the pellet with appropriate cryo-preservative media to freeze the sperm. In this method preserved sperms are free from natural antioxidant. Seminal plasma (separated) is a good source of antioxidant.
The Newly Designed Semen Extender.
The newly designed (New Extender For Preservation Of Human Spermatozoa) semen extender work well with both method of sperm preservation, whether raw or washed sperm. Certainly we use different concentration of constituent (particularly Glycerol) for raw sperm cryo-preservation and washed sperm preservation. The new extender for preservation of human spermatozoa is a mixture of sodium chloride, potassium chloride, sod. lactate, EDTA, taurine, KH2PO4, sod. bicarbonate, sucrose, glycine, hepes, gentamycin, glycerol. As a substitute of albumin (particularly human serum albumin) we used amino acids and sodium hyaluronate. Perhaps Amino acids works as nutrient , but its role is not clear at this point of time. But certainly amino acids is helpful to in post thaw recovery. Sodium hyaluronate defiantly reduce the stickiness of sperm with glass slide and cover slip and increase the maintenance of motility. We dont have any scope here to discuss Sodium hyaluronate role in capacitation. It increase the motility, particularly progressive forward motility. http://www.frozencell.org/blog/2018/07/10/hsa-in-human-sperm-freezing-media/
Discussion — My aim is to develop a new extender for the preservation of human spermatozoa. Which can be prepared anywhere without any variation. The present new extender contain all known chemicals. Therefore its action and reaction is more evident in comparison to HSA containing semen extender . With this newly developed extender there is all possibility to modify it according to the demand of situation or individual.
Preservation of human spermatozoa in a simple medium , Author links open overlay panel. Mohamed A.Dkhil. Abdallah R.Elgenaidi. Mohamed A.Danfour
Sodium hyaluronate, From Wikipedia, the free encyclopedia, https://en.wikipedia.org/wiki/Sodium_hyaluronate.
Semen Preservation , Steven P. Brinsko DVM, MS, PhD, Dipl ACT, … David L. HartmanDVM,