Why To Preserve Human Sperm with HSA ??

Human sperm preservation has been started in 1980. Much earlier  sperm preservation was being done for cattle with the intention to improve  pedigree.  Later on sperm preservation carried on for other mammalians, aquatics and avian also with different motives. During initial days of Intra-Uterine Insemination  (IUI) treatment only few disease was known as disease transmissible by IUI.  But now  we have a bigger list  of disease transmissible  by IUI.  Moreover  unidentified & emerging viruses  and  prions is ringing an alarm all the time

Keywords – Human sperm preservation,  Sperm preservation, Sperm Cryo-preservation, disease transmission by IUI.  Unidentified & emerging viruses, Prions.

Source of infection  in IUI  —

  1. Direct  Source    —————
  • Direct source are sperm donor /husband.
  • Sperm donor / husband can be properly screened before & after donation for any period of time.
  • Sperm donor or husband remain in direct contact with clinician or Sperm Bank,  therefore relatively easy to access  and screen.
  1. Indirect  Source   ———–

  • Indirect source are serum fraction (human, animal or avian products) added in Cryo-preservative media to augment sperm preservation (cryo-preservation), nutrition, post thaw motility enhancement etc.
  • Now a day mainly “Human serum albumin” (HSA) is used for human sperm preservation (cryo-preservation). It is being purchased from companies which is  not in direct contact with user.  Surprisingly even manufacturing company are giving dubious statement about  infectivity or possibility of transmission of disease. Manufactures are not in condition to give full assurance that disease will not be transmitted  by the used HSA.
  • Infertility clinic or clinician , who performs Human sperm preservation(cryo-preservation) do not have any direct control on quality of HSA. Even they can not cross check it.
  • Batch to batch variation in composition is common with HSA and  FBS
  • Rarely Fetus bovine serum (FBS) or egg yolk is used for Human sperm preservation (cryo-preservation). Zoonotic disease are coming up in sporadic manner all over world.  Over 200 diseases are currently classified as  zoonoses.   Intra Uterine Insemination by Human sperm preservation (cryo-preservation) may directly increase the incidence.
  • Serum is an ill defined  very complex mixture of large number of constituents  with multiple growth promoting & growth inhibiting activities
  • Serums contain different amount of endotoxins, hemoglobin & many other adverse factors  such as  bacteria, fungi,  viruses & prions.
  • Prions, if present with Bovine serum, can not be diagnosed or destroyed even at very high temperature .
  • Even for the manufacturing process of Vaccines many attempts are being taken to find a way to replace growth media containing bovine derived materials by “synthetic” media.
  • Freezing of sperm does not have any destructive effect on micro-organism / prions that is being freezed  along with sperm.

Purpose of discussion

The aim of this discussion is to point out theoretical & remote possibility of  disease transmission by IUI  through  human , animal or avian protein / serum.  Above all, possibility  of getting infected by unidentified & emerging viruses are always there.  For case of Prions all possible measures are being taken, but because of commercialization  & viability,  bovine serum manufacturing units may think to import  the animals.  And infected serum with prions can be supplied in the market.

                My point is – When  human sperm preservation (cryo-preservation)  can be performed without the use of  HAS  or any serum,  why to use  Human Serum Albumin (HSA)  or  any serum.

How effectively human sperm preservation (cryo-preservation) can be done even without  HSA   can  seen by  given short clip of video.  The used cryo-preservation media does not contain any complex molecule like HSA, or any  human, animal or avian protein. It is 100 % chemically defined  sperm  preservative (cryo-preservative) media.  Since it does not contain HSA,  therefore its  half life is more and cold chain maintenance is not mandatory but preferable.   It has been prepared simply by judicious use of amino acids and salts. Post thaw motility  recovery varies from 30 to 70 %. Progressive and straight forward motility is clearly visible  in the video clip.  As we experienced  progressive motility is relatively better with this newly developed HSA  free cryo-preservative media.

Reference

1/    Medical Definition of Zoonotic.
2/ Alternatives to the Use of Fetal Bovine Serum:  Serum-free Cell Culture,  Gerhard      Gstraunthaler,  Department of Physiology, University of Innsbruck, A-Innsbruck
3/  Archives of Andrology, Journal of Reproductive Systems,  Transmission of Sexually Transmitted Diseases by  Donor Semen,  B. S. Shanis, J. H. Check & A. F. Baker
4/    The European Agency for the Evaluation of Medicinal Products, Evaluation of Medicines for    Human Use,  London, 24 April 2001 EMEA/CPMP/BWP/819/01

3 Comments

  1. Frozen CellWorried About Azoospermia After Chemotherapy - Frozen Cell August 2, 2018 at 1:40 am

    […] Since  incidence of carcinoma is increasing in fertile age. Therefore  use of chemotherapy is getting more common .  Azoospermia After Chemotherapy means drastic reduction in sperm count after chemotherapy. Most importantly  Azoospermia After Chemotherapy is irreversible in most of the case.  Most of the time patients are  aware about No-Sperm After Chemotherapy or Azoospermia After Chemotherapy. The person undergoing chemotherapy and his  all near & dear one  expect  to have baby  even after chemotherapy. Further they also hope that preserved sperms must be 100% safe for future use.  For male here choice is to  Preserve Sperm without HSA.  http://www.frozencell.org/blog/2018/06/01/why-to-preserve-cryo-preserve-human-sperm-with-hsa-hsa-add… […]

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  2. Frozen CellNew Extender For Preservation Of Human Spermatozoa - Frozen Cell August 29, 2018 at 5:25 pm

    […] Unlike conventional semen extender, the New Extender For Preservation Of Human Spermatozoa is without albumin. Hence  does not contain any crude protein . Protein is a very complex molecule  among  all organic compounds.  Source of albumin , used for spermatozoa  preservation, is either  human or animal.  The newly developed New Extender For Preservation Of Human Spermatozoa does not contain albumin. Hence  the  New Extender For Preservation Of Human Spermatozoa does not have any bad effect of  protein (know or unknown) on spermatozoa.                                                        http://www.frozencell.org/blog/2018/06/01/why-to-preserve-cryo-preserve-human-sperm-with-hsa-hsa-add… […]

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  3. Worried About Azoospermia After Chemotherapy – Frozen Cell September 18, 2018 at 12:04 pm

    […] Since  incidence of carcinoma is increasing in fertile age. Therefore  use of chemotherapy is getting more common .  Azoospermia After Chemotherapy means drastic reduction in sperm count after chemotherapy. Most importantly  Azoospermia After Chemotherapy is irreversible in most of the case.  Most of the time patients are  aware about No-Sperm After Chemotherapy or Azoospermia After Chemotherapy. The person undergoing chemotherapy and his  all near & dear one  expect  to have baby  even after chemotherapy. Further they also hope that preserved sperms must be 100% safe for future use.  For male here choice is to  Preserve Sperm without HSA.  http://www.frozencell.org/blog/2018/06/01/why-to-preserve-cryo-preserve-human-sperm-with-hsa-hsa-add… […]

    Reply

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