Presently all sperm freezing media manufacturer of world are using either Human serum albumin or Fetal bovine serum or Egg yolk as a source of energy or to enhance cryopreservation of human sperm.
It is being claimed by them that used serum or protein is properly treated by different approved method and source materials used are found to be non-reactive to antibodies for Hepatitis B & C, human immunodeficiency virus etc. At the same time it Is also being warned all human source material should be treated as if it were capable of transmitting infection as the possibility of transmitting infective agents, including unknown or emerging viruses, cannot be totally excluded.
It is also being written by the sperm freezing media manufacturer — no test method offers complete assurance that products derived from human sources are noninfectious. Handle all human source material as if it were capable of transmitting infection. In the medical safety data sheet (MSDS) all companies state that they shall not be held liable for any damage resulting from handling or from contact with the product because it contain Human serum
There are reports that whole batch of Human serum albumin had been taken back from the market after detecting some infective materials.
Creutzfeldt-Jakob Disease (CJD) is giving its symptom even twenty years after infection. It is difficult to diagnose or detect carrier stage during initial period. There are reports, CJD had been diagnosed decades after using human / animal protein & patient could not be diagnosed earlier.
Bird flue / avian influenza is spreading all over world & causing morbidity & mortality. Recently diagnosed virus like Zika Virus is a recent threat, which yet need to be prevented.
Emerging viruses the biggest threat which will remain a challenge for medical science.
Serum may contain different amounts of endotoxins, haemoglobin, other adverse factors and a range of potentially bioactive molecules. Serum can be a potential source of microbial contaminants, such as fungi, bacteria, virus or prions.
There may be some ethical & religion reason to not accept Human or Animal protien. Fair chance to get infected as it is an excellent nutritional source to micro-organism. During transportation chance of getting infected is much higher, as cold chain may not be maintained in adverse condition.
With the above concept we have developed a new Sperm Cryopreservative media , which does not have any human, animal, bird any crude protein. It is 100% chemically defined. It contain amino acids, Sodium hyaluronate, glycerol, sucrose etc. In the term of post thaw recovery it is as good as presently used cryopreservative media. We are first in the world , going to preserve human sperm without any source of infection. It is a measure to prevent transmission of infection through the sperm when infertility particularly male infertility is increasing all over world.
This cryopreservative media may be also a model for cryopreservation of other tissue and organs.